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Sporl, F.; Korge, S.; Jurchott, K.; Wunderskirchner, M.; Schellenberg, K.; Heins, S.; Specht, A.; Stoll, C.; Klemz, R.; Maier, B.; Wenck, H.; Schrader, A.; Kunz, D.; Blatt, T.; Kramer, A. |
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Title |
Kruppel-like factor 9 is a circadian transcription factor in human epidermis that controls proliferation of keratinocytes |
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Journal Article |
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Year |
2012 |
Publication |
Proceedings of the National Academy of Sciences of the United States of America |
Abbreviated Journal |
Proc Natl Acad Sci U S A |
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Volume |
109 |
Issue |
27 |
Pages |
10903-10908 |
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Keywords |
Human Health; Anti-Inflammatory Agents/pharmacology; Biological Clocks/genetics/physiology; Cell Differentiation/physiology; Cell Proliferation/drug effects; Cells, Cultured; Circadian Rhythm/genetics/*physiology; Epidermis/cytology/*physiology; Genome-Wide Association Study; Homeostasis/physiology; Humans; Hydrocortisone/pharmacology; Keratinocytes/cytology/drug effects/*physiology; Kruppel-Like Transcription Factors/*genetics/*metabolism; Luciferases/genetics; Skin Neoplasms/genetics/physiopathology |
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Abstract |
Circadian clocks govern a wide range of cellular and physiological functions in various organisms. Recent evidence suggests distinct functions of local clocks in peripheral mammalian tissues such as immune responses and cell cycle control. However, studying circadian action in peripheral tissues has been limited so far to mouse models, leaving the implication for human systems widely elusive. In particular, circadian rhythms in human skin, which is naturally exposed to strong daytime-dependent changes in the environment, have not been investigated to date on a molecular level. Here, we present a comprehensive analysis of circadian gene expression in human epidermis. Whole-genome microarray analysis of suction-blister epidermis obtained throughout the day revealed a functional circadian clock in epidermal keratinocytes with hundreds of transcripts regulated in a daytime-dependent manner. Among those, we identified a circadian transcription factor, Kruppel-like factor 9 (Klf9), that is substantially up-regulated in a cortisol and differentiation-state-dependent manner. Gain- and loss-of-function experiments showed strong antiproliferative effects of Klf9. Putative Klf9 target genes include proliferation/differentiation markers that also show circadian expression in vivo, suggesting that Klf9 affects keratinocyte proliferation/differentiation by controlling the expression of target genes in a daytime-dependent manner. |
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Research and Development, Beiersdorf AG, 20245 Hamburg, Germany |
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English |
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0027-8424 |
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PMID:22711835; PMCID:PMC3390879 |
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no |
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LoNNe @ kagoburian @ |
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814 |
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Author |
van Diepen, H.C.; Foster, R.G.; Meijer, J.H. |
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Title |
A colourful clock |
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Journal Article |
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Year |
2015 |
Publication |
PLoS Biology |
Abbreviated Journal |
PLoS Biol |
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Volume |
13 |
Issue |
5 |
Pages |
e1002160 |
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Keywords |
Animals; Commentary; *Circadian Rhythm; suprachiasmatic nuclei; melanopsin; retinal ganglion cells; entrainment; photoperiod |
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Abstract |
Circadian rhythms are an essential property of life on Earth. In mammals, these rhythms are coordinated by a small set of neurons, located in the suprachiasmatic nuclei (SCN). The environmental light/dark cycle synchronizes (entrains) the SCN via a distinct pathway, originating in a subset of photosensitive retinal ganglion cells (pRGCs) that utilize the photopigment melanopsin (OPN4). The pRGCs are also innervated by rods and cones and, so, are both endogenously and exogenously light sensitive. Accumulating evidence has shown that the circadian system is sensitive to ultraviolet (UV), blue, and green wavelengths of light. However, it was unclear whether colour perception itself can help entrain the SCN. By utilizing both behavioural and electrophysiological recording techniques, Walmsley and colleagues show that multiple photic channels interact and enhance the capacity of the SCN to synchronize to the environmental cycle. Thus, entrainment of the circadian system combines both environmental irradiance and colour information to ensure that internal and external time are appropriately aligned. |
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Laboratory for Neurophysiology, Department of Molecular Cell Biology, Leiden University medical School, Leiden, The Netherlands |
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PLOS |
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English |
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English |
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1544-9173 |
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PMID:25996907; PMCID:PMC4440787 |
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LoNNe @ christopher.kyba @ |
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1183 |
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Bapary, M.A.J.; Takano, J.-I.; Soma, S.; Sankai, T. |
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Title |
Effect of blue LED light and antioxidants potential in a somatic cell |
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Journal Article |
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Year |
2019 |
Publication |
Cell Biology International |
Abbreviated Journal |
Cell Biol Int |
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in press |
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Keywords |
Cells; Biology |
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Abstract |
Light is an indispensable part of routine laboratory works in which conventional light is generally used. Light-emitting diodes (LEDs) have come to replace the conventional light thus could be a potent target in biomedical studies. Since blue light is a major component of visible light wavelength, in this study, using a somatic cell from African green monkey kidney, we assessed the possible consequences of blue spectra of LED light in future animal experiments and proposed a potent mitigation against light induced damages. COS-7 cells were exposed to blue LED light (450 nm) and the growth and DNA damage were assessed at different exposure times. A higher suppression in cell growth and viability was observed under a longer period of blue LED light exposure. The number of apoptotic cells increased as light exposure time was prolonged. Reactive oxygen species generation was also elevated in accordance to the extension of light exposure times. A comparison to dark-maintained cells revealed that the upregulation of ROS by blue LED light plays a significant role in causing cellular dysfunction in DNA in a time-dependent manner. In turn, antioxidant treatment has shown to improve the cell growth and viability under blue LED light conditions. This indicates that antioxidants are potential against blue LED light-induced somatic cell damage. It is expected that this study will contribute to the understanding of the basic mechanism of somatic cell death under visible light and to maximize the beneficial use of LED light in future animal experiments. This article is protected by copyright. All rights reserved. |
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Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Japan |
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English |
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1065-6995 |
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PMID:30958611 |
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Call Number |
GFZ @ kyba @ |
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2328 |
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