||Human activities are drastically changing the amount of artificial light entering natural habitats. Because light pollution alters the sensory environment, it may interfere with behaviors ranging from prey detection and vigilance to mate choice. Here, we test the hypothesis that anthropogenic light pollution affects the mate choice behavior of female Eastern Gray Treefrogs (Hyla versicolor). We tested this hypothesis under two experimental light treatments that simulate the light pollution created by streetlights (expansion of lit areas and increased light intensity), and the light pollution created by headlights of passing vehicles (rapid fluctuations between bright and dark conditions). The hypothesis predicts that females tested under conditions simulating light pollution will show behavioral changes geared toward mitigating detection by predators, such as relaxed preferences, decreased choosiness for the normally preferred call, and differences in approach behavior (either more directional, faster, or stealthier movements, or no approach at all). Contrary to our prediction, we found that light pollution did not affect mate choice behavior in Gray Treefrogs, and should therefore neither interfere with population persistence nor affect the sexual selection regimes on male call traits of this species. However, we caution that this result does not imply that anthropogenic light pollution is of no concern for amphibian conservation, because behavioral responses to variation in nocturnal light levels (both in the natural as well as anthropogenically enhanced range) seem to be highly species‐specific in anurans. We encourage additional studies to help gage the vulnerability of anurans to anthropogenic light pollution.