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Author (up) Nitta, Y.; Matsui, S.; Kato, Y.; Kaga, Y.; Sugimoto, K.; Sugie, A.
Title Analysing the evolutional and functional differentiation of four types of Daphnia magna cryptochrome in Drosophila circadian clock Type Journal Article
Year 2019 Publication Scientific Reports Abbreviated Journal Sci Rep
Volume 9 Issue 1 Pages 8857
Keywords Animals
Abstract Cryptochrome (CRY) plays an important role in the input of circadian clocks in various species, but gene copies in each species are evolutionarily divergent. Type I CRYs function as a photoreceptor molecule in the central clock, whereas type II CRYs directly regulate the transcriptional activity of clock proteins. Functions of other types of animal CRYs in the molecular clock remain unknown. The water flea Daphnia magna contains four Cry genes. However, it is still difficult to analyse these four genes. In this study, we took advantage of powerful genetic resources available from Drosophila to investigate evolutionary and functional differentiation of CRY proteins between the two species. We report differences in subcellular localisation of each D. magna CRY protein when expressed in the Drosophila clock neuron. Circadian rhythm behavioural experiments revealed that D. magna CRYs are not functionally conserved in the Drosophila molecular clock. These findings provide a new perspective on the evolutionary conservation of CRY, as functions of the four D. magna CRY proteins have diverse subcellular localisation levels. Furthermore, molecular clocks of D. magna have been evolutionarily differentiated from those of Drosophila. This study highlights the extensive functional diversity existing among species in their complement of Cry genes.
Address Brain Research Institute, Niigata University, Niigata, Japan. atsushi.sugie@bri.niigata-u.ac.jp
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 2045-2322 ISBN Medium
Area Expedition Conference
Notes PMID:31222139; PMCID:PMC6586792 Approved no
Call Number GFZ @ kyba @ Serial 2579
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