||Temperature, humidity and moisture content are the important abiotic component regulating seedling establishment in plants including rice. Light factor intensity and duration are also important environmental factors regulating rice growth and development. In the growth and development of rice crop germination followed by seedling establishment is the foremost and very important growth stages. Light component such as intensity, direction and duration are the regulating factor for several physiological, biochemical and molecular processes in plants. To consider these facts, in the present piece of work rice seed of HUR-105 grown under different light regimes, from T1 (2000 lux for 12 h during day time + 12 h dark period) (lower light intensity), T2 (4000 lux for 12 h during day time+ 12 h dark period), T3 (6000 lux for 12 h during day time + 12 h dark period) (moderate), T4 (9000 lux for 12 h during day time + 12 h dark period) (optimum) and to T5 (9000 lux for 12h during day time + 200 lux for 12 h during night time). Germination, seedling growth and biochemical parameters were observed at different time intervals. It was observed that germination %, germination index (GI), germination rate index (GRI), coefficient of velocity of germination (CVG), mean germination rate (MGR), seedling vigour (SV), α-amylase activity and soluble sugar content significantly reduced in both the treatments T1 and T5. Further, the mean time germination and insoluble sugar content were increased in T1 and T5 treatment. The present experiment concluded that both lower light intensity (T1) and addition of low light during dark period (considered as night light pollution) causes stress condition and reduce germination and seedling establishment potential of rice crop.