||Nighttime lighting is an increasingly important anthropogenic environmental stress on plants and animals. Exposure to unnatural lighting environments may disrupt circadian rhythm. However, studies involved in molecular biology, e.g. disruption of molecular circadian clock by light pollution, always have a small sample sizes. The small sample sizes result in a low statistical power and difficulties in replicating prior results. Here, a power-calibrated meta-analysis was developed to overcome these weakness. The results demonstrated that effect size of 2.48 in clock genes induced by artificial light would promised the reproducibility of the results as high as 80%. Long wavelength light entrained the positive core clock genes and negative core clock genes with robust circadian rhythmic expression, whereas some of those genes, e.g. cClock, cCry1, cCry2, cPer2, and cPer3, were arrhythmic in short wavelength light. Artificial light entrained the transcriptional-translational feedback loop of molecular clock in a wavelength-dependent manner. The expression positive core clock genes (cBmal1, cBmal2 and cClock), cAanat gene and melatonin were the greatest in short wavelength light and the lowest in long wavelength light. However, for negative regulators of molecular clock (cCry1, cCry2, cPer2 and cPer3), the greatest were in long wavelength light and the lowest were in short wavelength light. Our study opens up new opportunities to understand and strengthen conclusions based on the studies with small sample sizes and provides further insight about the disrupting in circadian rhythm by short wavelength light. Especially, the global lighting is shifting from “yellow” sodium lamps toward blue-enriched “white” light-emitting diodes (LEDs).