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Author (up) Agbaria, S.; Haim, A.; Fares, F.; Zubidat, A.E. url  doi
openurl 
  Title Epigenetic modification in 4T1 mouse breast cancer model by artificial light at night and melatonin – the role of DNA-methyltransferase Type Journal Article
  Year 2019 Publication Chronobiology International Abbreviated Journal Chronobiol Int  
  Volume Issue Pages 1-15  
  Keywords Human Health; Cosinor analysis; DNA methyltransferases; global DNA methylation; melatonin; splenomegaly; tissue-specific  
  Abstract Currently, one of the most disputed hypotheses regarding breast cancer (BC) development is exposure to short wavelength artificial light at night (ALAN) as multiple studies suggest a possible link between them. This link is suggested to be mediated by nocturnal melatonin suppression that plays an integral role in circadian regulations including cell division. The objective of the research was to evaluate effects of 1 x 30 min/midnight ALAN (134 micro Wcm(-2), 460 nm) with or without nocturnal melatonin supplement on tumor development and epigenetic responses in 4T1 tumor-bearing BALB/c mice. Mice were monitored for body mass (Wb) and tumor volume for 3 weeks and thereafter urine samples were collected at regular intervals for determining daily rhythms of 6-sulfatoxymelatonin (6-SMT). Finally, mice were sacrificed and the tumor, lungs, liver, and spleen were excised for analyzing the total activity of DNA methyltransferases (DNMT) and global DNA methylation (GDM) levels. Mice exposed to ALAN significantly reduced 6-SMT levels and increased Wb, tumor volume, and lung metastasis compared with controls. These effects were diminished by melatonin. The DNMT activity and GDM levels showed tissue-specific response. The enzymatic activity and GDM levels were lower in tumor and liver and higher in spleen and lungs under ALAN compared with controls. Our results suggest that ALAN disrupts the melatonin rhythm and potentially leading to increased BC burden by affecting DNMT activity and GDM levels. These data may also be applicable to early detection and management of BC by monitoring melatonin and GDM levels as early biomarker of ALAN circadian disruption.  
  Address b The Israeli Center for Interdisciplinary Research in Chronobiology , University of Haifa , Haifa , Israel; Zubidat3(at)013.net.il  
  Corporate Author Thesis  
  Publisher Taylor & Francis Place of Publication Editor  
  Language English Summary Language English Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0742-0528 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:30746962 Approved no  
  Call Number IDA @ john @ Serial 2211  
Permanent link to this record
 

 
Author (up) Agbaria, S.; Haim, A.; Fares, F.; Zubidat, A.E. url  doi
openurl 
  Title Epigenetic modification in 4T1 mouse breast cancer model by artificial light at night and melatonin – the role of DNA-methyltransferase Type Journal Article
  Year 2019 Publication Chronobiology International Abbreviated Journal Chronobiol Int  
  Volume in press Issue Pages  
  Keywords Animals  
  Abstract Currently, one of the most disputed hypotheses regarding breast cancer (BC) development is exposure to short wavelength artificial light at night (ALAN) as multiple studies suggest a possible link between them. This link is suggested to be mediated by nocturnal melatonin suppression that plays an integral role in circadian regulations including cell division. The objective of the research was to evaluate effects of 1 x 30 min/midnight ALAN (134 micro Wcm(-2), 460 nm) with or without nocturnal melatonin supplement on tumor development and epigenetic responses in 4T1 tumor-bearing BALB/c mice. Mice were monitored for body mass (Wb) and tumor volume for 3 weeks and thereafter urine samples were collected at regular intervals for determining daily rhythms of 6-sulfatoxymelatonin (6-SMT). Finally, mice were sacrificed and the tumor, lungs, liver, and spleen were excised for analyzing the total activity of DNA methyltransferases (DNMT) and global DNA methylation (GDM) levels. Mice exposed to ALAN significantly reduced 6-SMT levels and increased Wb, tumor volume, and lung metastasis compared with controls. These effects were diminished by melatonin. The DNMT activity and GDM levels showed tissue-specific response. The enzymatic activity and GDM levels were lower in tumor and liver and higher in spleen and lungs under ALAN compared with controls. Our results suggest that ALAN disrupts the melatonin rhythm and potentially leading to increased BC burden by affecting DNMT activity and GDM levels. These data may also be applicable to early detection and management of BC by monitoring melatonin and GDM levels as early biomarker of ALAN circadian disruption.  
  Address b The Israeli Center for Interdisciplinary Research in Chronobiology , University of Haifa , Haifa , Israel  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0742-0528 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:30746962 Approved no  
  Call Number GFZ @ kyba @ Serial 2212  
Permanent link to this record
 

 
Author (up) Alaimo, A.; Linares, G.G.; Bujjamer, J.M.; Gorojod, R.M.; Alcon, S.P.; Martinez, J.H.; Baldessari, A.; Grecco, H.E.; Kotler, M.L. url  doi
openurl 
  Title Toxicity of blue led light and A2E is associated to mitochondrial dynamics impairment in ARPE-19 cells: implications for age-related macular degeneration Type Journal Article
  Year 2019 Publication Archives of Toxicology Abbreviated Journal Arch Toxicol  
  Volume in press Issue Pages  
  Keywords Vision  
  Abstract Age-related macular degeneration (AMD) is a multifactorial retinal disease characterized by a progressive loss of central vision. Retinal pigment epithelium (RPE) degeneration is a critical event in AMD. It has been associated to A2E accumulation, which sensitizes RPE to blue light photodamage. Mitochondrial quality control mechanisms have evolved to ensure mitochondrial integrity and preserve cellular homeostasis. Particularly, mitochondrial dynamics involve the regulation of mitochondrial fission and fusion to preserve a healthy mitochondrial network. The present study aims to clarify the cellular and molecular mechanisms underlying photodamage-induced RPE cell death with particular focus on the involvement of defective mitochondrial dynamics. Light-emitting diodes irradiation (445 +/- 18 nm; 4.43 mW/cm(2)) significantly reduced the viability of both unloaded and A2E-loaded human ARPE-19 cells and increased reactive oxygen species production. A2E along with blue light, triggered apoptosis measured by MC540/PI-flow cytometry and activated caspase-3. Blue light induced mitochondrial fusion/fission imbalance towards mitochondrial fragmentation in both non-loaded and A2E-loaded cells which correlated with the deregulation of mitochondria-shaping proteins level (OPA1, DRP1 and OMA1). To our knowledge, this is the first work reporting that photodamage causes mitochondrial dynamics deregulation in RPE cells. This process could possibly contribute to AMD pathology. Our findings suggest that the regulation of mitochondrial dynamics may be a valuable strategy for treating retinal degeneration diseases, such as AMD.  
  Address Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Instituto de Quimica Biologica Ciencias Exactas y Naturales (IQUIBICEN), CONICET-Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428, Buenos Aires, Argentina. kotler@qb.fcen.uba.ar  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0340-5761 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:30778631 Approved no  
  Call Number GFZ @ kyba @ Serial 2229  
Permanent link to this record
 

 
Author (up) Bará, S.; Tapia, C.; Zamorano, J. url  doi
openurl 
  Title Absolute Radiometric Calibration of TESS-W and SQM Night Sky Brightness Sensors Type Journal Article
  Year 2019 Publication Sensors Abbreviated Journal Sensors  
  Volume 19 Issue 6 Pages  
  Keywords Instrumentation; calibration; SQM; TESS; photometer; sky brightness  
  Abstract We develop a general optical model and describe the absolute radiometric calibration of the readings provided by two widely-used night sky brightness sensors based on irradiance-to-frequency conversion. The calibration involves the precise determination of the overall spectral sensitivity of the devices and also the constant G relating the output frequency of the light-to-frequency converter chip to the actual band-weighted and field-of-view averaged spectral radiance incident on the detector (brightness). From these parameters, we show how to define a rigorous astronomical absolute photometric system in which the sensor measurements can be reported in units of magnitudes per square arcsecond with precise physical meaning.  
  Address Departmento Física Aplicada, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain; salva.bara(at)usc.es  
  Corporate Author Thesis  
  Publisher MDPI Place of Publication Editor  
  Language English Summary Language English Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1424-8220 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number IDA @ john @ Serial 2263  
Permanent link to this record
 

 
Author (up) Berman, S. url  doi
openurl 
  Title Opinion: Whither V(λ)? Type Journal Article
  Year 2019 Publication Lighting Research & Technology Abbreviated Journal Lighting Research & Technology  
  Volume 51 Issue 1 Pages 4-4  
  Keywords Vision  
  Abstract  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1477-1535 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number GFZ @ kyba @ Serial 2219  
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