Abstract: For many corals, the timing of broadcast spawning correlates strongly with a number of environmental signals (seasonal temperature, lunar, and diel cycles). Robust experimental studies examining the role of these putative cues in triggering spawning have been lacking until recently because it has not been possible to predictably induce spawning in fully closed artificial mesocosms. Here, we present a closed system mesocosm aquarium design that utilizes microprocessor technology to accurately replicate environmental conditions, including photoperiod, seasonal insolation, lunar cycles, and seasonal temperature from Singapore and the Great Barrier Reef (GBR), Australia. Coupled with appropriate coral husbandry, these mesocosms were successful in inducing, for the first time, broadcast coral spawning in a fully closed artificial ex situ environment. Four Acropora species (A. hyacinthus, A. tenuis, A. millepora, and A. microclados) from two geographical locations, kept for over 1 year, completed full gametogenic cycles ex situ. The percentage of colonies developing oocytes varied from ~29% for A. hyacinthus to 100% for A. millepora and A. microclados. Within the Singapore mesocosm, A. hyacinthus exhibited the closest synchronization to wild spawning, with all four gravid colonies releasing gametes in the same lunar month as wild predicted dates. Spawning within the GBR mesocosm commenced at the predicted wild spawn date but extended over a period of 3 months. Gamete release in relation to the time postsunset for A. hyacinthus, A. millepora, and A. tenuis was consistent with time windows previously described in the wild. Spawn date in relation to full moon, however, was delayed in all species, possibly as a result of external light pollution. The system described here could broaden the number of institutions on a global scale, that can access material for broadcast coral spawning research, providing opportunities for institutions distant from coral reefs to produce large numbers of coral larvae and juveniles for research purposes and reef restoration efforts.

Abstract: The position of the Moon in relation to the Earth and the Sun gives rise to several predictable cycles, and natural changes in nighttime light intensity are known to cause alterations to physiological processes and behaviors in many animals. The limited research undertaken to date on the physiological responses of animals to the lunar illumination has exclusively focused on the synodic lunar cycle (full moon to full moon, or moon phase) but the moon's orbit—its distance from the Earth—may also be relevant. Every month, the moon moves from apogee, its most distant point from Earth—and then to perigee, its closest point to Earth. Here, we studied wild barnacle geese (Branta leucopsis) to investigate the influence of multiple interacting lunar cycles on the physiology of diurnally active animals. Our study, which uses biologging technology to continually monitor body temperature and heart rate for an entire annual cycle, asks whether there is evidence for a physiological response to natural cycles in lunar brightness in wild birds, particularly “supermoon” phenomena, where perigee coincides with a full moon. There was a three‐way interaction between lunar phase, lunar distance, and cloud cover as predictors of nighttime mean body
temperature, such that body temperature was highest on clear nights when the full
moon coincided with perigee moon. Our study is the first to report the physiological responses of wild birds to “supermoon” events; the wild geese responded to the combination of two independent lunar cycles, by significantly increasing their body temperature at night. That wild birds respond to natural fluctuations in nighttime ambient light levels support the documented responses of many species to anthropogenic sources of artificial light, that birds seem unable to override. As most biological systems are arguably organized foremost by light, this suggests that any interactions between lunar cycles and local weather conditions could have significant impacts on the energy budgets of birds.

Abstract: When the visibility of an object or person in the roadway from a driver’s perspective is an issue, the potential effect of moonlight is sometimes questioned. To assess this potential effect, methods typically used to quantify visibility were performed during conditions with no moon and with a full moon. In the full moon condition, measurements were collected from initial moon rise until the moon reached peak azimuth. Baseline ambient light measurements of illumination at the test surface were measured in both no moon and full moon scenarios. Additionally, a vehicle with activated low beam headlamps was positioned in the testing area and the change in illumination at two locations forward of the vehicle was recorded at thirty-minute intervals as the moon rose to the highest position in the sky. Also, two separate luminance readings were recorded during the test intervals, one location 75 feet in front and to the left of the vehicle, and another 150 feet forward of the vehicle. These luminance readings yielding the change in reflected light attributable to the moon. In addition to the quantitative measurement of light contributed by the moon, documentation to the change in visibility of objects and pedestrians located on the roadway were documented through photographs. Calibrated nighttime photographs were taken from the driver’s perspective inside the vehicle with low beam headlamps activated. The photographs were analyzed after testing to determine how the light intensity of the pixels in the photographs changed at each thirty-minute interval due to the additional light contribution from the moon. The results of this testing indicate that the quantifiable change in visibility distance attributable to added moonlight was negligible, and in real-world driving situations, the effect of additional illumination from a full moon would be unlikely to affect the detection of an object or pedestrian in or near the travel lane of the roadway.