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Borck, P. C., Batista, T. M., Vettorazzi, J. F., Soares, G. M., Lubaczeuski, C., Guan, D., et al. (2018). Nighttime light exposure enhances Rev-erbalpha-targeting microRNAs and contributes to hepatic steatosis. Metabolism, 85, 250–258.
Abstract: OBJECTIVE: The exposure to artificial light at night (ALAN) disrupts the biological rhythms and has been associated with the development of metabolic syndrome. MicroRNAs (miRNAs) display a critical role in fine-tuning the circadian system and energy metabolism. In this study, we aimed to assess whether altered miRNAs expression in the liver underlies metabolic disorders caused by disrupted biological rhythms. RESULTS: We found that C3H/HePas mice exposed to ALAN developed obesity, and hepatic steatosis, which was paralleled by decreased expression of Rev-erbalpha and up-regulation of its lipogenic targets ACL and FAS in liver. Furthermore, the expression of Rev-erbalpha-targeting miRNAs, miR-140-5p, 185-5p, 326-5p and 328-5p were increased in this group. Consistently, overexpression of these miRNAs in primary hepatocytes reduced Rev-erbalpha expression at the mRNA and protein levels. Importantly, overexpression of Rev-erbalpha-targeting miRNAs increased mRNA levels of Acly and Fasn. CONCLUSION: Thus, altered miRNA profile is an important mechanism underlying the disruption of the peripheral clock caused by exposure to ALAN, which could lead to hepatic steatosis.